Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Positron Emission Tomography01:29

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body being...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The retinal nerve fibre layer thickness slope: a localised biomarker of the structure-function relationship in early glaucoma.

The British journal of ophthalmology·2025
Same author

Dynamic contrast in dual-beam optical coherence tomography imaging of human skin.

Biomedical optics express·2025
Same author

Analysis on optical coherence tomography images to detect irregularities and restoration on paper-based artwork.

Optics express·2025
Same author

Introduction to the Biophotonics Congress 2024 feature issue.

Biomedical optics express·2025
Same author

Image Quality in Adaptive Optics Optical Coherence Tomography of Diabetic Patients.

Diagnostics (Basel, Switzerland)·2025
Same author

Standardization of OCT Angiography Nomenclature in Retinal Vascular Diseases: Consensus-Based Recommendations.

Ophthalmology. Retina·2025
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Sample motion-insensitive, full-range, complex, spectral-domain optical-coherence tomography.

Stefan Zotter1, Michael Pircher, Erich Götzinger

  • 1Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Währingerstrasse 13, A-1090 Vienna, Austria. stefan.zotter@meduniwien.ac.at

Optics Letters
|December 3, 2010
PubMed
Summary
This summary is machine-generated.

We developed a novel spectral-domain optical-coherence-tomography (SD-OCT) system using a double-beam scanning approach. This advanced imaging technique is insensitive to sample motion, enabling clear visualization of biological tissues like the optic nerve head and teeth.

More Related Videos

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

Related Experiment Videos

Last Updated: Jun 6, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

Area of Science:

  • Biomedical Optics
  • Medical Imaging Technology
  • Ophthalmology and Dentistry Applications

Background:

  • Spectral-domain optical coherence tomography (SD-OCT) is a vital imaging modality for high-resolution cross-sectional visualization of biological tissues.
  • Existing SD-OCT systems can be susceptible to motion artifacts, limiting imaging quality and diagnostic accuracy, particularly in vivo.
  • Complex signal reconstruction in SD-OCT typically requires precise phase information, which can be challenging to obtain reliably.

Purpose of the Study:

  • To introduce a novel full-range, complex spectral-domain optical-coherence-tomography (SD-OCT) system.
  • To develop a system that overcomes the limitations of sample motion artifacts inherent in conventional SD-OCT.
  • To demonstrate the system's capability for high-quality in vivo imaging of biological structures.

Main Methods:

  • Implementation of a double-beam scanning approach by combining two identical SD-OCT setups collinearly.
  • Utilizing a bulk optic beam splitter to merge sample beams before object illumination.
  • Achieving complex signal reconstruction through the phase difference between the two interferometers, inherently compensating for sample motion.

Main Results:

  • The developed double-beam scanning SD-OCT system demonstrated complete insensitivity to sample motion.
  • High-quality in vivo images of the human optic nerve head were successfully acquired.
  • Detailed images of a human tooth were also obtained, showcasing the system's versatility.

Conclusions:

  • The novel double-beam scanning SD-OCT system offers a robust solution for motion-artifact-free imaging.
  • This technology holds significant potential for enhanced diagnostic imaging in ophthalmology and dentistry.
  • The system's ability to provide complex, full-range data without motion artifacts represents a substantial advancement in OCT technology.