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[Determination of matrine in matrine injection by microfluidic chip with contactless conductivity detection]
Chong Wang1, Yong-Chong Li, Zi-You Cai
1Guangdong Food and Drug Vocational College, Guangzhou 510520, China. wangc@gdyzy.edu.cn
Objective:
To establish a new method for the determination of matrine in matrine injection by microfluidic chip with contactless conductivity detection.
Methods:
The electrophoretic parameters, such as the variety and concentration of buffer solution and additive, separation voltage and injection time, etc. were researched.
Results:
In the optimal conditions, using 2 mmol/L HAc + 2 mmol/L NaAc (pH 4. 5) as buffer solution, with 0.20 mmol/L SDS as addition at the separation voltage of 2.00 kV and 10s injection, matrine was separated and detected within 2 min. The linear dependence of the concentration of matrine ranged from 10 to 300 microg/mL (r2 = 0.9992), with the detection limit of 1.0 microg/mL (S/N = 3), the RSD of 1.9% and the recoveries from 98.7% to 101%.
Conclusion:
The method is simple, rapid and well reproducible, and it can be used in the quality control of the product.
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