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Updated: Jun 5, 2026

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
Published on: June 14, 2024
Interspecific polymorphism of gene lipL32 restriction profiles of pathogenic leptospires
A N Vaganova1, O A Freilikhman, N A Stoyanova
1Pasteur Institute of Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being, St. Petersburg, Russia. yersinia@land.ru
Abstract:
We analyzed restriction fragment length polymorphism of lipL32 gene encoding outer membrane protein in three Leptospira genomic species (L. interrogans, L. kirschneri, and L. borgpetersenii) using Bsa29 I and Bam HI restriction endonucleases. It was found that restriction profiles of the studied gene were similar at both the intraspecific and serogroup levels; variability was revealed only at the interspecific level, which can be explained by relatively low variability of lipL32 gene.

