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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

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In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
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Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
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[IDENTIFICATION OF LEPTOSPIRA SEROVARS BY MALDI-TOF MASS-SPEC- TROMETRY].

E V Zyeva, N A Stoyanova, N K Tokarevich

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |January 30, 2019
    PubMed
    Summary
    This summary is machine-generated.

    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows promise for identifying Leptospira serovars. This method accurately matched isolates to reference strains, suggesting potential for rapid leptospirosis diagnostics.

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    Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

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    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Analytical Chemistry

    Background:

    • Leptospirosis is a significant zoonotic disease caused by pathogenic Leptospira bacteria.
    • Accurate identification of Leptospira serovars is crucial for epidemiological surveillance and disease control.
    • Current serological methods for Leptospira typing can be laborious and time-consuming.

    Purpose of the Study:

    • To evaluate the potential of MALDI-TOF mass spectrometry for the identification of Leptospira isolates at the serovar level.
    • To establish a rapid and reliable method for Leptospira serovar typing.

    Main Methods:

    • MALDI-TOF mass spectrometry was employed to analyze 8 reference Leptospira serovars and 11 clinical isolates.
    • Main Spectral Profiles (MSP) were created for reference strains.
    • Isolates were identified by comparing their mass spectra to the MSP database using matching rate coefficients.

    Main Results:

    • MALDI-TOF MS successfully differentiated between Leptospira serovars.
    • Isolates belonging to Pomona, Icterohaemorrhagiae, and Canicola serogroups showed spectral similarities to saprophyte strain L. biflexa Patoc I, likely due to shared O-antigen peaks.
    • High matching rate coefficients accurately correlated with the serovar type of the isolates.

    Conclusions:

    • MALDI-TOF mass spectrometry is a viable method for identifying Leptospira serovars.
    • This technique offers a potential for developing a simple, rapid diagnostic tool for leptospirosis causative agents.
    • Further research can optimize this method for routine clinical and epidemiological applications.