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Updated: Jun 5, 2026
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Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
Published on: February 27, 2019
[Quantitative chimerism analysis of regulatory T cell subsets based on immunomagnetic sorting].
Xian-Guo Xu1, Wei Zhang, Xiao-Zhen Hong
1Zhejiang Provincial Blood Center, Hangzhou, 310006, Zhejiang Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi
|December 24, 2010
Summary
This study establishes a method for quantitative chimerism analysis of regulatory T (Treg) cells. The technique accurately detects chimerism from 1% to 10%, aiding transplant monitoring.
Area of Science:
- Immunology
- Genetics
Context:
- Monitoring chimerism is crucial after allogeneic hematopoietic stem cell transplantation.
- Regulatory T (Treg) cells play a key role in immune regulation post-transplant.
Purpose:
- To assess the feasibility of quantitative chimerism analysis in Treg cells.
- To develop a method combining immune sorting and short tandem repeat (STR) analysis for Treg cells.
Summary:
- Artificial chimera samples were created by mixing lymphocytes at varying proportions.
- CD4(+)CD25(+) Treg cells were isolated using immunomagnetic beads.
- Short tandem repeat (STR) polymorphisms at 16 loci were analyzed in sorted Treg cells.
- The method demonstrated sensitivity and accuracy for detecting chimerism between 1% and 10%.
Impact:
- A reliable method for quantitative chimerism analysis of Treg cells has been established.
- This technique can be applied to monitor hematopoietic chimerism after transplantation.
- The findings support improved patient monitoring and management in transplantation settings.
