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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Published on: April 26, 2017
Alternative splicing in stem cell self-renewal and diferentiation
1Department of Cellular and Molecular Medicine, Stem Cell Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, California, 92037, USA.
Advances in Experimental Medicine and Biology
|January 12, 2011
Summary
Alternative pre-messenger RNA splicing is crucial in stem cell biology, regulating RNA abundance and proteome diversity. New technologies allow genome-wide assessment of splicing during stem cell differentiation.
Area of Science:
- Molecular Biology
- Stem Cell Biology
- Genetics
Background:
- Alternative pre-messenger RNA splicing is a key post-transcriptional regulatory mechanism.
- Splicing generates proteome diversity and controls RNA abundance.
- Its role in stem cell biology is an area of active research.
Purpose of the Study:
- To review recent advances in understanding alternative splicing in stem cell biology.
- To highlight key alternative splicing events in stem cells.
- To provide an overview of technologies for genome-wide splicing assessment.
Main Methods:
- Review of current literature on alternative splicing and stem cell biology.
- Discussion of specific examples of alternative splicing events.
- Overview of microarray and sequencing technologies for splicing analysis.
Main Results:
- Alternative splicing significantly impacts stem cell biology.
- Several key alternative splicing events have been identified in stem cells.
- Advanced technologies enable comprehensive genome-wide splicing studies.
Conclusions:
- Alternative splicing is a critical regulator in stem cell differentiation.
- Systematic, genome-wide assessment of splicing is now feasible.
- Further research will elucidate the full impact of splicing on stem cell fate.
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