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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

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Related Experiment Video

Updated: Jun 5, 2026

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro
10:45

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro

Published on: September 11, 2008

Dielectrophoretic tweezer for isolating and manipulating target cells.

A Menachery1, D Graham, S M Messerli

  • 1Institute for Integrated Micro and Nano Systems, Joint Research Institute for Integrated Systems, School of Engineering, Edinburgh, University of Edinburgh, Edinburgh EH9 3JF, UK.

IET Nanobiotechnology
|January 19, 2011
PubMed
Summary

Researchers developed a new dielectrophoretic (DEP) tweezer for precise 3D single-cell manipulation. This accessible technology aids in isolating and relocating specific cells for biological research.

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

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Last Updated: Jun 5, 2026

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro
10:45

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Published on: September 11, 2008

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Area of Science:

  • Cell biology
  • Biophysics
  • Bioengineering

Background:

  • Precise 3D manipulation of single cells is crucial for advancing biological research.
  • Existing methods for cell isolation and relocation can be complex and costly.
  • Dielectrophoretic (DEP) tweezers offer a promising non-invasive approach for cell handling.

Purpose of the Study:

  • To design, theoretically model, and test a novel dielectrophoretic (DEP) tweezer.
  • To create an accessible DEP tweezer using standard electrophysiology laboratory equipment.
  • To improve upon existing DEP tweezer designs for practical cell manipulation.

Main Methods:

  • Theoretical modeling of the dielectrophoretic forces involved in cell manipulation.
  • Construction of the DEP tweezer using readily available laboratory materials.
  • Experimental testing using transfected HEI-193 human schwannoma cells.
  • Utilizing green fluorescent protein (GFP) for visual identification of target cells.

Main Results:

  • The novel DEP tweezer successfully isolates and relocates single target cells in three dimensions.
  • The device is constructible with standard electrophysiology lab equipment, reducing cost and complexity.
  • The design offers practical advantages over previously reported DEP tweezer systems.
  • Successful cell manipulation was demonstrated using GFP-labeled HEI-193 cells.

Conclusions:

  • The developed DEP tweezer provides an effective and accessible tool for 3D single-cell isolation and relocation.
  • This technology can be readily adopted in standard biological laboratories.
  • The DEP tweezer facilitates advanced cell-based research requiring precise cell positioning.