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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
Transcriptional and Posttranscriptional Programming by Long Noncoding RNAs.
Radha Raman Pandey1, Chandrasekhar Kanduri
1Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Dag Hammarskjölds Väg 20, 75185, Uppsala, Sweden.
Progress in Molecular and Subcellular Biology
|February 3, 2011
Summary
Noncoding RNAs (ncRNAs), especially long noncoding RNAs (lncRNAs), are abundant in eukaryotes. Their roles in development and differentiation are being uncovered through epigenetic and nonepigenetic mechanisms.
Area of Science:
- Molecular Biology
- Genetics
- Epigenetics
Background:
- Noncoding RNAs (ncRNAs) constitute a significant portion of eukaryotic transcriptomes.
- The functional roles of ncRNAs, particularly long noncoding RNAs (lncRNAs), in development and differentiation remain largely undefined.
- Emerging evidence suggests lncRNAs participate in crucial biological functions at transcriptional and posttranscriptional levels.
Purpose of the Study:
- To discuss the mechanisms underlying the involvement of lncRNAs in biological functions.
- To explore both epigenetic and nonepigenetic pathways regulated by lncRNAs and their transcription.
- To provide insights into the programming of biological functions by lncRNAs across diverse model systems.
Main Methods:
- Review of existing literature on lncRNA function.
- Analysis of epigenetic mechanisms influenced by lncRNAs.
- Examination of nonepigenetic regulatory roles of lncRNAs.
Main Results:
- lncRNAs and their transcription are implicated in regulating gene expression.
- Both epigenetic modifications and nonepigenetic factors are involved in lncRNA-mediated functions.
- Evidence spans from yeast to mammalian model systems, indicating conserved roles.
Conclusions:
- lncRNAs play critical roles in organismal development and differentiation.
- Understanding lncRNA mechanisms is essential for deciphering complex biological processes.
- Further research into lncRNA functions will illuminate fundamental aspects of eukaryotic biology.
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