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Updated: Jun 4, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
Sugar-bearing tetraphenylethylene: novel fluorescent probe for studies of carbohydrate-protein interaction based on
Jin-Xiang Wang1, Qi Chen, Ning Bian
1National Center for Nanoscience and Technology, Beijing 100190, China.
Abstract:
Neutral sugar-bearing tetraphenylethenes (TPE) are designed and prepared as "turn-on" luminescent sensors for lectins and glycosidases based on aggregation-induced emission. Through aggregation derived from carbohydrate-lectin binding, multivalent mannosyl-bearing TPE shows a good selectivity and sensitivity to Con A by switching on the fluorescence of water-soluble tetraphenylethylene-based glyco-conjugates in aqueous solution. Meanwhile, cellobiosyl-bearing TPE can be used to investigate enzymatic hydrolysis based on emission enhancing by glycosidase-induced aggregation.
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