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Ultrasensitive fluorescence polarization DNA detection by target assisted exonuclease III-catalyzed signal

Min Zhang1, Yi-Meng Guan, Bang-Ce Ye

  • 1Lab of Biosystems and Microanalysis, East China University of Science & Technology, Meilong RD 130, Shanghai, 200237, China.

Chemical Communications (Cambridge, England)
|February 10, 2011
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...