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Flurbiprofen in human crevicular fluid analyzed by high-performance liquid chromatography
P A Heasman1, A Ward, A W Barrett
1Dental School, University of Newcastle upon Tyne, U.K.
Journal of Periodontal Research
|March 1, 1990
Summary
Flurbiprofen, an anti-inflammatory drug, is detectable in human crevicular fluid (CF) after oral administration. This finding is significant for understanding its potential in treating periodontal disease.
Area of Science:
- Pharmacology
- Periodontology
- Analytical Chemistry
Background:
- Periodontal disease involves alveolar bone loss.
- Flurbiprofen is a non-steroidal anti-inflammatory drug (NSAID) known to inhibit bone loss.
- Understanding drug presence in crevicular fluid (CF) is crucial for localized treatment efficacy.
Purpose of the Study:
- To determine if orally administered flurbiprofen is present in human CF.
- To quantify flurbiprofen concentrations in CF and serum.
- To assess the relationship between serum and CF drug levels.
Main Methods:
- Developed a high-performance liquid chromatography (HPLC) method for detecting flurbiprofen in small CF volumes (20 µL).
- Induced experimental gingivitis in volunteers to increase CF flow.
- Administered 100 mg oral flurbiprofen and collected serum and CF samples over time.
Main Results:
- Flurbiprofen was detected in human CF following oral dosing.
- Peak CF concentrations on Day 21 were 0.32 µg/mL at 4 hours post-dose.
- On Day 28, peak serum concentration was 13.13 µg/mL and CF concentration was 0.46 µg/mL, with CF levels remaining relatively constant.
Conclusions:
- Oral flurbiprofen administration leads to its detectable presence in human crevicular fluid.
- CF drug levels may exceed plasma levels, suggesting potential for localized therapeutic effects in periodontal disease.
- The developed HPLC method allows for sensitive detection of flurbiprofen in CF.