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Frozen water phase method for logD measurement using a 96-well plate
Taro Yamashita1, Eiichi Yamamoto, Ikuo Kushida
1Analytical Research, CMC Japan, Pharmaceutical Science & Technology Function Unit, Eisai Co. Ltd., 1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan. t5-yamashita@hhc.eisai.co.jp
Talanta
|April 13, 2011
Summary
A novel frozen water phase method prevents octanol contamination during logD measurements. This accurate technique is effective for lipophilic compounds and evaluates pH-logD profiles across a wide range.
Area of Science:
- Analytical Chemistry
- Physical Chemistry
Background:
- Octanol contamination is a significant challenge in traditional logD measurements, particularly for lipophilic compounds.
- Contamination of the water phase by the octanol phase can strongly influence measured concentrations.
Purpose of the Study:
- To develop a novel, accurate, and convenient method for logD measurement that overcomes octanol contamination.
- To establish a reliable method for evaluating the pH-logD profile of compounds.
Main Methods:
- A frozen water phase method utilizing a 96-well plate was developed.
- The water phase was frozen in liquid nitrogen, allowing for the removal of the unfrozen octanol phase.
- Washing steps with octanol and a water/ethanol mixture were employed to eliminate residual octanol.
Main Results:
- The method demonstrated validity for commercially available drugs across a logD range of 0 to 4.
- The technique effectively evaluated pH-logD profiles for compounds between pH 2 and pH 12.
- The developed method proved convenient and accurate, preventing contamination effectively.
Conclusions:
- The frozen water phase method offers a robust solution to octanol contamination in logD measurements.
- This technique provides a wide dynamic range and is suitable for diverse compound lipophilicity.
- The method facilitates accurate determination of pH-dependent lipophilicity, crucial for drug development.
