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Updated: Jun 2, 2026

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Published on: March 24, 2014
STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution
Mike Friedrich1, Qiang Gan, Vladimir Ermolayev
1Molecular Microscopy Group, Bio-Imaging Center, Rudolf Virchow Center, University of Würzburg, Würzburg, Germany.
Abstract:
We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The integrated STED-SPIM method combines the advantages of SPIM with the resolution enhancement of STED, and thus provides a method for fast, high-resolution imaging with >100 μm deep penetration into biological tissue.
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