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Updated: Jun 2, 2026

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
14:06

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

Colorimetric SNP genotyping based on allele-specific PCR by using a thiol-labeled primer

Ye Lim Jung1, Cheulhee Jung, Harshala Parab

  • 1Department of Chemical and Biomolecular Engineering (BK 21 program), KAIST, Yuseong-gu, Daejeon, Republic of Korea.

Chembiochem : a European Journal of Chemical Biology
|May 11, 2011
PubMed
Abstract

No abstract available in PubMed .

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...