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Published on: March 3, 2015
Paternity testing in a PBL environment.
Alberto Vicario Casla1, Isabel Smith Zubiaga
1Departmento de Genética, Antropología Física y Fisiología Animal, Facultad de Ciencia y Tecnología. Universidad del País Vasco. Apdo. 644. 48080 Bilbao, Spain. alberto.vicario@ehu.es, isabel.smith@ehu.es.
This Problem Based Learning (PBL) activity uses paternity testing to teach genetics. Students apply PCR techniques to analyze DNA markers, calculate allele frequencies, and assess Hardy-Weinberg equilibrium, enhancing their understanding of molecular genetics and forensic science applications.
Area of Science:
- Genetics
- Molecular Biology
- Pedagogy
Background:
- Problem Based Learning (PBL) enhances student engagement by using real-world scenarios.
- Paternity testing provides a relevant context for applying genetic principles.
Purpose of the Study:
- To present a Problem Based Learning (PBL) module for paternity testing.
- To integrate classroom, laboratory, and independent learning activities.
- To evaluate student learning outcomes through various assessment methods.
Main Methods:
- Students designed a PCR-based protocol to determine their genotypes for two genetic markers.
- Class-wide genotype data were used to calculate allelic frequencies.
- Hardy-Weinberg equilibrium was assessed using pooled class data.
- Individual results were analyzed for paternity assessment.
Main Results:
- The PBL approach successfully engaged students in designing and executing genetic analysis protocols.
- Students gained practical experience in PCR, genotyping, and population genetics calculations.
- The activity facilitated understanding of genetic principles and their application in paternity testing.
Conclusions:
- Paternity testing via PBL is an effective method for teaching molecular genetics and forensic applications.
- The integrated approach of classroom, lab, and out-of-class activities supports comprehensive learning.
- Diverse evaluation methods ensure a thorough assessment of student learning and outcomes.
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