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Related Experiment Video

Updated: Jun 2, 2026

Tissue Engineering of the Intestine in a Murine Model
08:45

Tissue Engineering of the Intestine in a Murine Model

Published on: December 1, 2012

Murine tissue-engineered stomach demonstrates epithelial differentiation.

Allison L Speer1, Frederic G Sala, Jamil A Matthews

  • 1Developmental Biology and Regenerative Medicine Program, The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California 90027, USA.

The Journal of Surgical Research
|May 17, 2011
PubMed
Summary
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Scientists engineered a functional stomach in mice, successfully regenerating gastric glands and all four cell types. This tissue-engineered stomach shows promise for future human stomach replacement therapies.

Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Gastroenterology

Background:

  • Gastric cancer is a leading cause of cancer mortality globally, with significant post-surgical morbidity.
  • Current treatments for gastric cancer lead to poor quality of life due to impaired gastric function.
  • Tissue-engineered stomach offers a potential solution for restoring gastric reservoir and physiological functions.

Purpose of the Study:

  • To investigate the development of tissue-engineered stomach in a mouse model.
  • To evaluate epithelial differentiation, proliferation, and stem cell marker expression in engineered stomach tissue.
  • To establish a foundation for future human stomach replacement therapies.

Main Methods:

  • Organoid units from young mouse stomachs were seeded onto biodegradable scaffolds.

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Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
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Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

Published on: May 7, 2018

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
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Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

Published on: May 18, 2015

Related Experiment Videos

Last Updated: Jun 2, 2026

Tissue Engineering of the Intestine in a Murine Model
08:45

Tissue Engineering of the Intestine in a Murine Model

Published on: December 1, 2012

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
11:48

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

Published on: May 7, 2018

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
12:16

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

Published on: May 18, 2015

  • Constructs were implanted into the omentum of adult mice.
  • Histology and immunohistochemistry were used to analyze harvested implants.

    • Main Results:

    • Engineered stomach formed an expanding sphere with columnar epithelium and gastric glands.
    • Regenerated epithelium exhibited differentiation of mucous, enteroendocrine, chief, and parietal cells.
    • Epithelial proliferation rates matched native stomach, with expression of stem cell markers DCAMKL-1 and Lgr5.

    Conclusions:

    • Successfully generated tissue-engineered stomach in a mouse model for the first time.
    • Demonstrated appropriate proliferation and differentiation of regenerated gastric epithelium.
    • Provides a murine model for studying regenerative mechanisms, crucial for human stomach replacement therapy.