Related Experiment Video
Updated: Jun 1, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Identification of bacteria using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Mollie G Kedney1, Kevin B Strunk, Lisa M Giaquinto
1Department of Chemistry, Lebanon Valley College, Annville, Pennsylvania 17003-1400.
Abstract:
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS or simply MALDI)1 has become ubiquitous in the identification and analysis of biomacromolecules. As a technique that allows for the molecular weight determination of otherwise nonvolatile molecules, MALDI has had a profound impact in the molecular biosciences, especially in the field of proteomics. MALDI analysis of whole organisms allows for the generation of a phenotypic profile or "molecular fingerprint;" once established, these fingerprints can be used for identification purposes.
More Related Videos
07:49Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Published on: September 9, 2013
11:09Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)
Published on: January 2, 2015
Related Concept Videos
Rapid Identification of Pathogens
Matrix-Assisted Laser Desorption Ionization (MALDI)
MALDI-TOF Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Tandem Mass Spectrometry
Mass Spectrum: Interpretation