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Published on: February 22, 2019
[Serological activity of vaccine and circulating Bordetella pertussis strains]
Insights
Pertussis antibodies in infected and vaccinated children showed similar activity against both vaccine and circulating Bordetella pertussis strains. However, vaccinated children
Area of Science:
- Immunology
- Microbiology
- Vaccinology
Background:
- Pertussis remains a significant public health concern, necessitating ongoing evaluation of vaccine efficacy.
- Understanding the immune response to Bordetella pertussis is crucial for improving vaccination strategies.
Purpose of the Study:
- To assess the serological activity of vaccine and circulating Bordetella pertussis strains.
- To compare antibody responses in children with pertussis infection versus healthy vaccinated children.
Main Methods:
- Hemagglutination assay (HA), radial gel immunodiffusion (RGI), and immunoelectrophoresis (IEP) were employed.
- Serum samples from 146 infected and 158 vaccinated children (aged 3-5 years) were analyzed.
- Reactions were tested against 3 vaccine strains and 7 circulating B. pertussis strains.
Main Results:
- Both infected and vaccinated children's sera showed equal agglutination activity against vaccine and circulating strains in HA.
- RGI revealed significant reactivity in infected children's sera (81-85%) against both strain types.
- Vaccinated children's sera showed lower but comparable reactivity (16-20%) against both strain types in RGI.
Conclusions:
- Antibodies in pertussis patients are equally active against vaccine and circulating Bordetella pertussis strains.
- Antibodies in vaccinated children also exhibit similar activity but recognize a narrower antigen spectrum compared to infected children.
Aim:
To study activity of vaccine and circulating strains of Bordetella pertussis in serological reactions with serum samples from healthy vaccinated children and children with pertussis infection.
Materials And Methods:
One hundred forty-six serum samples from children with pertussis infection as well as 158 samples from healthy vaccinated children aged 3 - 5 years old were studied. Serologic activity of 3 vaccine strains and 7 strains of B. pertussis isolated from patients with pertussis in 2001 - 2005 against sera of children with pertussis infection or vaccinated children was assessed with hemagglutination assay (HA), radial gel immunodiffusion (RGI), and immunoelectrophoresis (IEP).
Results:
In HA both serum samples of infected and vaccinated children were equally active in agglutination of microbial preparations prepared from vaccine or recently isolated strains of B. pertussis. RGI assay showed that 81 - 84% of serum samples from infected children and 17 -19% of samples from healthy vaccinated children reacted with vaccine strains, and 81 - 85% of samples from infected children and 16 - 20% of samples from healthy vaccinated children reacted with circulating strains: Sera from patients with pertussis formed identical lines of precipitation with vaccine and circulating strains in RGI assay and three types of precipitation arches profile in IEP. Sera from healthy vaccinated children formed identical precipitation arches with vaccine and circulating strains in RGI assay and one type of precipitation arches profile in IEP.
Conclusion:
Antibodies of patients with pertussis were equally active against vaccine and circulating strains of B. pertussis. Antibodies of vaccinated children were also equally active against vaccine and circulating strains although revealed more narrow spectrum of antigens compared to children with pertussis infection.
