[PROFILES OF CYTOKINES IN MICE DURING IMMUNIZATION WITH ADTP- VACCINE WITH ACELLULAR PERTUSSIS COMPONENT]

Area of Science:

• Immunology
• Vaccinology

Background:

• Pertussis remains a significant public health concern, necessitating effective vaccination strategies.
• Acellular pertussis vaccines (aPV) and adsorbed pertussis-diphtheria-tetanus (aDTaP) vaccines are widely used for pertussis prevention.
• Understanding the cytokine profiles induced by these vaccines is crucial for assessing their immunogenicity.

Purpose of the Study:

• To investigate the cytokine responses in mice following immunization with acellular pertussis vaccine (aPV) and adsorbed pertussis-diphtheria-tetanus (aDTaP) vaccines.
• To compare the cytokine profiles induced by aPV and aDTaP vaccines.

Main Methods:

• Mice were immunized intraperitoneally with aPV or aDTaP vaccines.
• Cytokine levels (IFN-γ, IL-2, IL-4, IL-5, IL-12) were measured using enzyme immunoassay after each immunization.
• Intact mice served as a control group.

Main Results:

• Immunization with both aPV and aDTaP vaccines led to an increase in IFN-γ, IL-2, IL-5, and IL-12 levels.
• No significant stimulation of IL-4 production was observed.
• These changes occurred dynamically after each immunization dose.

Conclusions:

• Both aPV and aDTaP vaccines effectively activate the production of cytokines associated with cellular and humoral immune responses.
• The observed cytokine profiles are characteristic of immune responses during pertussis infection and vaccination with whole-cell vaccines.
• These findings support the immunogenicity of acellular pertussis vaccines.