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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

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Microscopic laser desorption/postionization fourier transform mass spectrometry.

J M Behm1, J C Hemminger, K R Lykke

  • 1Materials Science and Chemistry Divisions, Argonne National Laboratory, Argonne, Illinois 60439, and Department of Chemistry and Institute for Surface and Interface Science, University of California, Irvine, California 92717.

Analytical Chemistry
|May 31, 2011
PubMed
Summary

A new microscopic laser desorption/postionization Fourier transform mass spectrometer (LD/FTMS) offers sub-micrometer resolution for materials analysis. This technique enhances sensitivity and selectivity for characterizing atoms and molecules at the microscale.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Spectroscopy

Background:

  • Traditional mass spectrometry methods often lack the spatial resolution required for detailed microscale materials characterization.
  • Achieving high mass resolution and high lateral resolution simultaneously presents a significant analytical challenge.

Purpose of the Study:

  • To describe a novel microscopic laser desorption/postionization Fourier transform mass spectrometer (LD/FTMS).
  • To demonstrate the capability of this instrument for high-resolution, sensitive, and selective microscale materials characterization.

Main Methods:

  • Development and implementation of a microscopic laser desorption/postionization Fourier transform mass spectrometer (LD/FTMS).
  • Utilizing laser postionization to enhance sensitivity and selectivity.
  • Leveraging Fourier transform mass spectrometry for high mass resolution.

Main Results:

  • Achieved lateral resolution of less than 1 micrometer (<1 μm).
  • Maintained inherent high mass resolution of Fourier transform mass spectrometry.
  • Demonstrated successful microscopic desorption and postionization of atoms and molecules.

Conclusions:

  • The developed LD/FTMS system provides unprecedented lateral resolution for mass spectrometry.
  • The technique enables sensitive and selective characterization of materials at the micrometer scale.
  • This advancement opens new possibilities for diverse materials analysis applications.