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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 1, 2026

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

High-throughput multispot single-molecule spectroscopy.

Ryan A Colyer1, Giuseppe Scalia, Taiho Kim

  • 1Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA.

Proceedings of Spie--The International Society for Optical Engineering
|June 7, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a faster method for single-molecule spectroscopy and fluorescence correlation spectroscopy (FCS) using parallelized multispot excitation and detection. This approach significantly reduces acquisition times for molecular property analysis.

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Last Updated: Jun 1, 2026

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Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

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Area of Science:

  • Biophysical Chemistry
  • Spectroscopy
  • Nanotechnology

Background:

  • Solution-based single-molecule spectroscopy and fluorescence correlation spectroscopy (FCS) are vital for determining molecular properties.
  • Current limitations include low concentrations and long acquisition times for statistical accuracy.

Purpose of the Study:

  • To develop a parallelized approach for faster data acquisition in single-molecule spectroscopy and FCS.
  • To overcome the limitations of low concentrations and lengthy measurement durations.

Main Methods:

  • Utilized a multispot excitation and detection geometry.
  • Employed a liquid crystal spatial light modulator for multiple excitation spots.
  • Integrated a multipixel detector array and a reconfigurable multichannel counting board.

Main Results:

  • Demonstrated a novel multispot excitation and detection system.
  • Successfully performed fluorescence correlation spectroscopy (FCS) measurements on calibrated samples.
  • Acquired single-molecule burst data efficiently.

Conclusions:

  • The developed multispot technique enables rapid data acquisition in single-molecule spectroscopy and FCS.
  • This parallelized approach enhances efficiency for analyzing molecular properties.
  • The system offers a promising advancement for biophysical studies.