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Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
Published on: October 4, 2011
Electrospray deposition as a method to fabricate functionally active protein films.
1W. M. Keck Foundation Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, New York, New York 10003, and Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia.
Analytical Chemistry
|June 14, 2011
Summary
Electrospray deposition can create active protein films. Complete enzyme activity preservation during electrospray deposition (ESD) was achieved using low current, humidity, and protective disaccharides.
Area of Science:
- Biochemistry
- Materials Science
- Analytical Chemistry
Background:
- Electrospray ionization (ESI) is a standard technique for analyzing biopolymers like proteins.
- Developing methods to create functional protein films from ESI is an area of interest.
- Assessing protein intactness after electrospray deposition (ESD) is crucial for functional applications.
Purpose of the Study:
- To investigate the feasibility of manufacturing functionally active protein films using electrospray deposition.
- To determine the factors affecting protein intactness and activity during ESD.
- To optimize ESD parameters for complete preservation of enzyme activity.
Main Methods:
- Electrospray deposition (ESD) of alkaline phosphatase (AP) onto a conductive electrode.
- Assessing the recovery of specific hydrolytic activity of electrosprayed AP as a measure of protein intactness.
- Systematically varying voltage, current, humidity, and the presence of protective substances (disaccharides).
Main Results:
- Protein inactivation during ESD is significantly influenced by voltage and current.
- Humidity and the absence of protective agents in the solution negatively impact enzyme activity.
- Complete preservation of alkaline phosphatase activity was achieved under specific low-current and low-humidity conditions when disaccharides were present.
Conclusions:
- Electrospray deposition is a viable method for producing functionally active protein films.
- Controlling ESD parameters like current and humidity, along with using protective agents such as disaccharides, is essential for maintaining protein integrity.
- This technique offers a promising route for fabricating active protein-based materials.
