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Ca2+ channel blockade inhibits gallbladder ion transport
K D Saunders1, J A Cates, M Z Abedin
1Research Service, Sepulveda Veterans Administration Medical Center, California.
The Journal of Surgical Research
|October 1, 1990
Summary
Calcium channel blockers like verapamil significantly inhibit gallbladder ion transport, suggesting intracellular calcium regulates this process. This finding may impact understanding of gallstone formation.
Area of Science:
- Physiology
- Gastroenterology
- Pharmacology
Background:
- Gallstone (GS) formation involves altered gallbladder (GB) epithelial ion transport and increased luminal Ca2+.
- Intracellular Ca2+ is a known modulator of intestinal ion transport.
Purpose of the Study:
- To investigate the effects of Ca2+ channel inhibition on gallbladder ion transport.
Main Methods:
- Prairie dog GBs were mounted in a Ussing chamber.
- Short-circuit current (Isc), transepithelial potential difference (Vms), and tissue resistance (Rt) were measured.
- Verapamil (VER) was applied to the mucosal surface.
Main Results:
- Verapamil rapidly inhibited Isc by 76% at 1 mM (P < 0.001) and decreased Vms (P < 0.001).
- A significant increase in tissue resistance (Rt) was observed (P < 0.05).
- Effects were dose-dependent and largely reversible.
Conclusions:
- Ca2+ channel blockade with verapamil inhibits gallbladder ion transport.
- Intracellular Ca2+ may play a regulatory role in gallbladder ion transport.
- Findings contribute to understanding gallstone formation mechanisms.