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Related Concept Videos

Synthesis and Regulation of Thyroid Hormones01:20

Synthesis and Regulation of Thyroid Hormones

Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
Functions of Thyroid Hormones01:18

Functions of Thyroid Hormones

The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...

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Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

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Published on: March 25, 2009

TSH receptor function is required for normal thyroid differentiation in zebrafish.

Robert Opitz1, Emilie Maquet, Maxime Zoenen

  • 1Institute of Interdisciplinary Research in Molecular Human Biology, Université Libre de Bruxelles, Brussels, Belgium.

Molecular Endocrinology (Baltimore, Md.)
|July 9, 2011
PubMed
Summary
This summary is machine-generated.

Thyroid-stimulating hormone receptor (TSHR) signaling is crucial for functional thyroid differentiation in zebrafish. Disrupting TSHR impairs follicle development and thyroid hormone synthesis, highlighting zebrafish as a model for vertebrate thyroid studies.

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Area of Science:

  • Endocrinology and Developmental Biology
  • Comparative Vertebrate Development
  • Molecular Endocrinology

Background:

  • Thyroid-stimulating hormone (TSH) regulates thyroid function via its receptor (TSHR).
  • Understanding TSHR's role in thyroid organogenesis and differentiation is essential.
  • Zebrafish offer a valuable model for studying vertebrate development and endocrine functions.

Purpose of the Study:

  • To investigate functional thyroid differentiation in zebrafish.
  • To characterize the role of TSHR signaling during zebrafish thyroid organogenesis.
  • To identify novel thyroid differentiation markers in zebrafish.

Main Methods:

  • Cloned zebrafish Tshr cDNA and confirmed conserved properties with mammalian TSHR.
  • Used in situ hybridization to analyze tshr expression patterns during development.
  • Utilized morpholino microinjection to knockdown Tshr function and assessed phenotypic effects on thyroid development and differentiation markers.

Main Results:

  • Identified tpo, iyd, duox, and duoxa as novel zebrafish thyroid differentiation markers.
  • TSHR knockdown affected later functional differentiation, reducing follicle number/size and down-regulating differentiation markers, but not early morphogenesis.
  • Revealed conserved thyroid-enriched expression of B-cell lymphoma 2 family regulators in zebrafish and mouse during early thyroid morphogenesis.

Conclusions:

  • TSHR signaling is critical for later stages of functional thyroid differentiation in zebrafish.
  • Zebrafish serve as an effective model for studying vertebrate thyroid development and TSHR function.
  • The findings enhance understanding of the molecular mechanisms governing thyroid organogenesis and functional differentiation.