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[Pathway analysis of the differential expression genes of oligonucleotide microarray of airway allergic diseases
Jinmei Xue1, Changqing Zhao, Aihua Liang
1Department of Otolaryngology, the Second Hospital of Shanxi Medical University, Shanxi, 030001, China.
Objective:
To detected the mechanism of allergic rhinitis associated with asthma with bioinformatics methods.
Method:
GenMAPP software was used to analyze the expression profile of nasal mucosa of seasonal allergic rhinitis(SAR) and SAR associated with asthma of oligonucleotide microarray (Affymetrix HG-U133-plus2). One the first step,of differentially expressed genes screening were done, then differential gene database retrieval was established, at last pathway analysis was performed.
Result:
689 genes out of 47 000 analyzed transcripts of nasal mucosa of SAR associated with asthma were differentially expressed at least 4-fold, in which 233 genes were up regulated and 456 genes were down regulated. These differential expression genes participate in 69 bio-pathways, in which the interaction pathway between cytokine and cytokine receptor was most. Chemotactic factor CXCL12 and its receptor CXCR4 expressed in SAR associated with asthma patients were up-regulated predominantly, compared with that in SAR patients.
Conclusion:
Multiple pathways were involved in the development of SAR and SAR complicated with asthma. The CXCL12/CXCR4 axis might play a main role in the allergic airway diseases.
