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[HPLC fingerprint of Pericarpium Citri Reticultae from Guangdong province]
Yang Yang1, Lin Jiang, Guo-dong Zheng
1Laboratory of Pharmacognosy and Natural Medicine Chemistry, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. yyabc1007@126.com
Objective:
To establish chromatographic fingerprint of Pericarpium Citri Reticultae from Guangdong province by HPLC and comparison of citrus herbs similarity with different strains and different origin, it was expected to fix standard of quality control.
Methods:
26 samples' analysis was performed on Diamonsil C18 column (250 mm x 4.6 mm, 5 microm) with gradient mobile phase of acetonitrile-1% acetic acid,fingerprint was finished in 65 min, the monitoring wavelength was at 330 nm with the flow rate of 1.0 mL/min.
Results:
Establishing the fingerprint of Pericarpium Citri Reticultae from Guangdong province, 17 common peaks were found in the HPLC fingerprints from different sources, the similarity of Pericarpium Citri Reticultae from Guangdong province above 0.975 and quite different for different origin drugs.
Conclusion:
This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Pericarpium Citri Reticultae from Guangdong province.
