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Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
Published on: January 6, 2018
Differentiation of mouse bone marrow derived stem cells toward microglia-like cells
Arnd Hinze1, Alexandra Stolzing
1Fraunhofer Institute for Cell Therapy and Immunology (IZI), Perlickstrasse 1, 04103, Leipzig, Germany.
BMC Cell Biology
|August 23, 2011
Summary
Bone marrow stem cells can differentiate into microglia, the brain's immune cells. These lab-grown microglia are functional and could be used for cell therapy in neurodegenerative diseases.
Area of Science:
- Neuroscience
- Immunology
- Stem Cell Biology
Background:
- Microglia, brain macrophages, are crucial in neurodegeneration and aging.
- Microglial replenishment via resident cell proliferation is known; bone marrow origin is debated.
- Investigated differentiation of mouse microglia from bone marrow (BM) stem cells.
Purpose of the Study:
- To investigate the differentiation of mouse microglia from bone marrow stem cells.
- To assess the effects of FMS-like tyrosine kinase 3 ligand (Flt3L), astrocyte-conditioned medium (ACM), and GM-CSF on microglia differentiation.
- To evaluate the functional capacity and migratory potential of in vitro-derived microglia.
Main Methods:
- Assessed in vitro-derived microglia differentiation using marker expression (CD11b/CD45, F4/80).
- Evaluated functional performance including phagocytosis and oxidative burst.
- Assessed in situ migration into living brain tissue within organotypic brain slices.
Main Results:
- Differentiated cells from mouse BM exhibited markers, morphology, and function of primary microglia.
- These cells demonstrated migration into living brain tissue.
- Flt3L negatively impacted differentiation, while GM-CSF enhanced it.
Conclusions:
- In vitro-derived microglia are phenotypic and functional equivalents of primary microglia.
- These cells hold potential for therapeutic applications in cell therapy.
- Further research can explore their use in treating neurodegenerative conditions.
