Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

13.5K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.5K
Methods of reducing fever01:22

Methods of reducing fever

1.6K
The signs and symptoms of fever include hot and dry skin, flushed face, thirst, muscle aches, anorexia, headache, tachycardia, tachypnea, and fatigue. Elevated body temperature is reduced using two methods: pharmacological and nonpharmacological. Proper identification and treatment of the root cause of a fever is of utmost importance.
Pharmacological Methods of Reducing Fever:
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipid- and Polymer-Based Nanoscale Delivery of Anti-Aging Phytochemicals.

Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology·2026
Same author

A review of ageing related biomarkers in breath.

Ageing research reviews·2026
Same author

Open problems in ageing science: a roadmap for biogerontology.

GeroScience·2025
Same author

Invigorating discovery and clinical translation of aging biomarkers.

Nature aging·2025
Same author

Longevity biotechnology: bridging AI, biomarkers, geroscience and clinical applications for healthy longevity.

Aging·2024
Same author

A systematic review of lifespan studies in rodents using stem cell transplantations.

Ageing research reviews·2024

Related Experiment Video

Updated: May 3, 2026

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.4K

New Cryoprotectants for Cell Therapies.

Alexandra Stolzing1

  • 1Centre for Biological Engineering, Wolfson School of Mechanical and Manufacturing Engineering, Loughborough University, Loughborough, UK. A.Stolzing@lboro.ac.uk.

Advances in Experimental Medicine and Biology
|October 24, 2025
PubMed
Summary
This summary is machine-generated.

This chapter explores cryoprotective agents (CPAs) for cell cryopreservation, focusing on cell therapy applications. It reviews new CPA classes and innovative discovery strategies like machine learning.

Keywords:
Anti-freeze proteinsApoptosisCell deathCell therapyCryoprotectantsDimethylsulfoxideFreezingIce formationMachine learningMetabolitesModelingNanomaterialsOxidative stressPolymersShelf-life

More Related Videos

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation
04:10

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation

Published on: October 20, 2023

2.1K
Author Spotlight: Development of an Efficient and Feasible Method of Retinal Pigment Epithelial Cell Freezing
07:03

Author Spotlight: Development of an Efficient and Feasible Method of Retinal Pigment Epithelial Cell Freezing

Published on: November 3, 2023

1.1K

Related Experiment Videos

Last Updated: May 3, 2026

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.4K
Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation
04:10

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation

Published on: October 20, 2023

2.1K
Author Spotlight: Development of an Efficient and Feasible Method of Retinal Pigment Epithelial Cell Freezing
07:03

Author Spotlight: Development of an Efficient and Feasible Method of Retinal Pigment Epithelial Cell Freezing

Published on: November 3, 2023

1.1K

Area of Science:

  • Biotechnology and Biomedical Engineering
  • Cell Biology and Tissue Engineering

Background:

  • Cryopreservation is crucial for cell therapy, requiring effective cryoprotective agents (CPAs) to maintain cell viability.
  • Traditional CPAs have limitations, necessitating the development of novel agents.

Purpose of the Study:

  • To explore the role of CPAs in cell cryopreservation, with an emphasis on cell therapy applications.
  • To provide an overview of emerging CPA classes, including nanomaterials, metabolites, and polymers.
  • To highlight innovative research strategies for discovering novel CPAs.

Main Methods:

  • Literature review of existing and emerging CPAs.
  • Exploration of novel CPA categories: nanomaterials, metabolites, and polymers.
  • Discussion of advanced research methodologies, including computational modeling and machine learning for CPA discovery.

Main Results:

  • Identification of new classes of CPAs with potential for improved cryoprotection.
  • Highlighting the utility of nanomaterials, metabolites, and polymers as CPAs.
  • Demonstration of the effectiveness of modeling and machine learning in accelerating CPA discovery.

Conclusions:

  • Novel CPAs are essential for advancing cell therapy through improved cryopreservation.
  • Emerging CPA classes and innovative discovery methods show significant promise.
  • The integration of computational approaches is key to future CPA development.