Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Regulation of Metabolism01:19

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
Allosteric Regulation01:08

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
Allosteric Regulation01:08

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromatin architecture sets origin licensing capacity.

Research square·2026
Same author

Chromatin architecture sets origin licensing capacity.

bioRxiv : the preprint server for biology·2026
Same author

Proteasome-dependent degradation and nucleus-vacuole junctions sustain proteostasis during acute glucose starvation.

bioRxiv : the preprint server for biology·2026
Same author

Roles of RRM2 and RRM2B in pyrimidine stress responses and differentiation of acute myeloid leukemia cells.

Cell death discovery·2026
Same author

Sir2 and Fun30 regulate ribosomal DNA replication timing via MCM helicase positioning and nucleosome occupancy.

eLife·2025
Same author

Utilization of primary tumor samples for cancer neoantigen discovery.

Journal for immunotherapy of cancer·2025
Same journal

Endothelial Cell Phenotypic Plasticity in Atherosclerosis.

Handbook of experimental pharmacology·2026
Same journal

Endothelial Dysfunction and Neurovascular Alterations in Autism Spectrum Disorder.

Handbook of experimental pharmacology·2026
Same journal

Molecular Mechanisms of Endothelial Shear Stress Mechanotransduction in Health and Disease.

Handbook of experimental pharmacology·2026
Same journal

Microvasculature of the Pancreatic Islets of Langerhans in Health and Diabetes.

Handbook of experimental pharmacology·2026
Same journal

Mechanisms of Actions of Physiological, Pharmacological, and Toxicological Dietary Bioactive Inorganic Boron.

Handbook of experimental pharmacology·2026
Same journal

BNCT Plus Luminescence: New Paradigm for Boron-Containing Drug Design.

Handbook of experimental pharmacology·2026
See all related articles

Related Experiment Video

Updated: May 29, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Sirtuin modulators.

Sumit S Mahajan1, Vid Leko, Julian A Simon

  • 1Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

Handbook of Experimental Pharmacology
|September 1, 2011
PubMed
Summary
This summary is machine-generated.

Sirtuin enzymes, like Sir2, extend lifespan in simple organisms. Research now explores sirtuin activators and inhibitors for treating diverse age-related diseases, offering new therapeutic targets.

Related Experiment Videos

Last Updated: May 29, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Gerontology

Background:

  • Sirtuin proteins, including the founding Sir2 in yeast, are associated with lifespan extension in simple organisms.
  • This link has spurred research into sirtuin orthologues' roles in aging-related conditions such as neurodegeneration, type II diabetes, and cancer.

Purpose of the Study:

  • To review the development of pharmacological small molecule activators and inhibitors targeting the sirtuin family of enzymes.
  • To highlight sirtuins as attractive targets for developing therapeutic agents for aging-associated diseases.

Main Methods:

  • Review of existing literature on sirtuin research.
  • Analysis of studies investigating genetic and pharmacologic manipulation of sirtuin activity.
  • Examination of the development of small molecule modulators (activators and inhibitors) of sirtuins.

Main Results:

  • Genetic and pharmacologic manipulation of sirtuin activity shows beneficial effects across a wide range of aging-associated conditions.
  • The role of sirtuin manipulation (activation vs. inhibition) is context-dependent, varying with the specific disease and target tissue.
  • Small molecule activators and inhibitors of sirtuins have been developed.

Conclusions:

  • Sirtuin enzymes represent a promising target for pharmacological interventions in aging-related diseases.
  • Therapeutic strategies may involve either activating or inhibiting sirtuin activity, depending on the specific condition.
  • The development of small molecule modulators offers potential for novel treatments for age-associated pathologies.