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Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Gas Chromatography: Types of Detectors-I01:21

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...

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Updated: May 29, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Hydrodynamic chromatography: packed columns, multiple detectors, and microcapillaries.

André M Striegel1

  • 1Department of Chemistry & Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USA. striegel@chem.fsu.edu

Analytical and Bioanalytical Chemistry
|September 9, 2011
PubMed
Summary
This summary is machine-generated.

Hydrodynamic chromatography (HDC) separates molecules by size in solution, with larger ones eluting first. This technique is regaining popularity for particle sizing and macromolecular analysis, offering insights into size, shape, and molar mass.

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Physical Chemistry

Background:

  • Hydrodynamic chromatography (HDC) separates analytes based on their size in solution.
  • Separations occur in open tubes or packed columns with nonporous beads.
  • Larger analytes elute first, smaller ones later, due to flow streamline sampling.

Purpose of the Study:

  • To review the resurgence and applications of HDC.
  • To highlight HDC's utility in particle sizing and macromolecular analysis.
  • To discuss information gained from multi-detector combinations in HDC.

Main Methods:

  • Hydrodynamic chromatography (HDC) principles of separation.
  • Application of HDC in open tubes and packed columns.
  • Utilizing multiple detection methods for comprehensive analysis.

Main Results:

  • HDC provides information on molar mass, size, shape, and compactness.
  • Microcapillary HDC is increasingly used in bioanalysis, separating DNA fragments over four orders of magnitude.
  • Examples demonstrate HDC's versatility across various scientific domains.

Conclusions:

  • HDC offers valuable insights into molecular properties.
  • The technique is experiencing a revival due to its versatility and information-rich outputs.
  • Future applications of HDC, particularly microcapillary formats, are promising.