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Updated: May 29, 2026
05:47
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
Sequential and exhaustive ionization of analytes with different surface activity by probe electrospray ionization
Mridul Kanti Mandal1, Lee Chuin Chen, Kenzo Hiraoka
1Clean Energy Research Center, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan.
Journal of the American Society for Mass Spectrometry
|September 29, 2011
Summary
Probe electrospray ionization (PESI) separates analytes by surface activity. This technique allows for distinct detection of compounds like detergents, proteins, and lipids in complex samples over time.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Surface Science
Background:
- Probe electrospray ionization (PESI) is an established ESI-based technique.
- Understanding analyte behavior in PESI is crucial for complex sample analysis.
Purpose of the Study:
- To investigate the temporal elution order of analytes in single-shot PESI.
- To determine if analyte surface activity influences detection sequence.
Main Methods:
- Mass spectrometry analysis of single-shot PESI.
- Time-resolved measurement of analyte elution from a mixture.
- Analysis of human breast cancer tissue using PESI.
Main Results:
- Analytes elute in order of decreasing surface activity.
- Detergents and proteins were detected sequentially in a mixed sample.
- Temporal separation of proteins and lipids observed in breast cancer tissue analysis.
Conclusions:
- PESI exhibits a suppression effect based on surface activity.
- This allows for sequential detection of different analyte classes.
- PESI offers an advantage for analyzing complex biological samples with distinct components.
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