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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Increasing phosphoproteomic coverage through sequential digestion by complementary proteases.

Jason M Gilmore1, Arminja N Kettenbach, Scott A Gerber

  • 1Department of Genetics, Dartmouth Medical School, Lebanon, NH 03756, USA.

Analytical and Bioanalytical Chemistry
|October 18, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a sequential digestion method using Glu-C and trypsin proteases to improve phosphoproteomic analysis. This new approach enhances coverage of the human lung cancer phosphoproteome, identifying new phosphoproteins and phosphorylation sites.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Area of Science:

  • Biochemistry
  • Proteomics
  • Cancer Research

Background:

  • Protein phosphorylation is a key post-translational modification regulating cellular processes.
  • Dysregulated phosphorylation is common in cancers like non-small-cell lung adenocarcinoma.
  • Current phosphoproteomic methods face challenges in sensitivity and coverage.

Purpose of the Study:

  • To develop an improved method for phosphoproteomic characterization.
  • To increase the coverage of the human lung cancer phosphoproteome.
  • To identify novel phosphoproteins and phosphorylation sites.

Main Methods:

  • Development of a sequential digestion workflow using complementary proteases (Glu-C and trypsin).
  • Evaluation of the method's productivity and orthogonality compared to traditional workflows.
  • Comparison of results with existing public phosphoproteomic databases.

Main Results:

  • The sequential digestion method significantly increases phosphoproteomic coverage.
  • This approach is more productive than workflows using only Glu-C.
  • New phosphoproteins and phosphorylation sites were identified in the human lung cancer phosphoproteome.

Conclusions:

  • The sequential digestion method enhances phosphoproteomic characterization.
  • This technique provides access to previously inaccessible regions of the phosphoproteome.
  • The method offers valuable insights for kinomic studies and cancer biomarker discovery.