Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 26, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Characterizing in situ Glycerophospholipids with SIMS and MALDI Methodologies.

M K Passarelli¹, N Winograd

¹Department of Chemistry, The Pennsylvania State University, 104 Chemistry Building, University Park, Pennsylvania 16802, USA.

Surface and Interface Analysis : SIA

|December 23, 2011

Summary

This study combines MALDI and SIMS techniques for detailed lipid analysis, overcoming challenges like salt contamination and isobaric interference to identify lipids in neurons.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Investigating the Fundamentals of Molecular Depth Profiling Using Strong-field Photoionization of Sputtered Neutrals.

Surface and interface analysis : SIA·2015

Same author

Ionization effects in molecular depth profiling of trehalose films using buckminsterfullerene (C<sub>60</sub>) cluster ions.

Surface and interface analysis : SIA·2013

Same author

Fundamental studies of molecular depth profiling using organic delta layers as model systems.

Surface and interface analysis : SIA·2013

Same author

Temperature effects in the sputtering of a molecular solid by energetic atomic and cluster projectiles.

Surface and interface analysis : SIA·2013

Same author

Strong-field Photoionization of Sputtered Neutral Molecules for Molecular Depth Profiling.

The journal of physical chemistry. C, Nanomaterials and interfaces·2010

Same author

Imaging macrophages in trehalose with SIMS.

Applied surface science·2010

Area of Science:

Analytical Chemistry
Biochemistry
Neuroscience

Background:

Characterizing lipids in situ presents challenges, including isobaric interference and salt contamination.
Complex lipid profiles require advanced analytical techniques for accurate identification.

Purpose of the Study:

To evaluate the dual functionality of a C(60) (+)-Q-Star hybrid instrument for lipid profiling.
To address challenges in in situ lipid characterization using combined methodologies.
To establish characteristic fragmentation pathways for complex lipids.

Main Methods:

Utilized Matrix-Assisted Laser Desorption/Ionization (MALDI) and Secondary Ion Mass Spectrometry (SIMS) methodologies.
Employed a C(60) (+)-Q-Star hybrid instrument for dual analysis.

More Related Videos

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

Related Experiment Videos

Last Updated: May 26, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

Leveraged high lateral resolution of SIMS for single-cell imaging.

Main Results:

Successfully deconvoluted complex lipid spectra by combining MALDI and SIMS advantages.
Established characteristic fragmentation pathways for key lipid components.
Generated single-cell images of aplysia californica neurons using SIMS.

Conclusions:

The hybrid MALDI-SIMS approach effectively overcomes in situ lipid analysis challenges.
This method enables detailed lipid profiling and identification at the single-cell level.
The study identified a major lipid component in aplysia californica neurons.