Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-Assembly of Stimuli-Responsive Peptide Enhances Therapeutics by Specifically Disrupting Hepatocellular Carcinoma Lysosomes In Vivo.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

A Multi-Indicator Fusion-Based Technique for the Identification of Acoustic Emission Signals During Rock Failure.

Sensors (Basel, Switzerland)·2026
Same author

Comprehensive experimental study on the key performance of a semi-submersible aquaculture cage.

iScience·2026
Same author

A Dual-Layer MOF Microneedle Patch for Self-Sensitized Mild Photothermal Therapy and Autophagy Regulation.

Advanced healthcare materials·2026
Same author

Geometric Preorganization Enables Entropy-Constrained Proton Migration for Ultrafast and Stable Aqueous Proton Batteries.

Angewandte Chemie (International ed. in English)·2026
Same author

Mechanisms and Clinical Significance of Endosomal Toll-Like Receptors in Neurological Diseases.

Molecular neurobiology·2025

Related Experiment Video

Updated: May 26, 2026

Analysis of Epididymal Protein Synthesis and Secretion
10:23

Analysis of Epididymal Protein Synthesis and Secretion

Published on: August 25, 2018

Prostaglandin E synthase is regulated in postnatal mouse testis.

Yanjun Zhang1, Yi Du, Dengyu Wang

  • 1Institute of Developmental Biology, School of Life Sciences, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, Shandong University, Jinan 250100, PR China.

Frontiers in Bioscience (Elite Edition)
|December 29, 2011
PubMed
Summary

Cyclic prostaglandin E synthase (cPGES) expression increases in mouse testes during development, correlating with spermatogenesis. High cPGES levels in mature sperm cells suggest a role in regulating male fertility.

More Related Videos

In Vivo Microinjection and Electroporation of Mouse Testis
08:39

In Vivo Microinjection and Electroporation of Mouse Testis

Published on: August 23, 2014

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
09:47

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Published on: October 21, 2015

Related Experiment Videos

Last Updated: May 26, 2026

Analysis of Epididymal Protein Synthesis and Secretion
10:23

Analysis of Epididymal Protein Synthesis and Secretion

Published on: August 25, 2018

In Vivo Microinjection and Electroporation of Mouse Testis
08:39

In Vivo Microinjection and Electroporation of Mouse Testis

Published on: August 23, 2014

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
09:47

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Published on: October 21, 2015

Area of Science:

  • Reproductive Biology
  • Molecular Endocrinology
  • Developmental Biology

Background:

  • Prostaglandins are crucial signaling molecules in the male reproductive system.
  • Cyclic prostaglandin E synthase (cPGES) is a key enzyme in prostaglandin synthesis.

Purpose of the Study:

  • To investigate the expression pattern and regulation of cPGES during postnatal development in mouse testes.
  • To understand the role of cPGES in Sertoli cells, spermatocytes, and spermatids.

Main Methods:

  • Analysis of cPGES expression levels in mouse testes at various postnatal developmental stages (5, 10, 15, and 20 days, and adulthood).
  • Localization of cPGES expression in different testicular cell types (Sertoli cells, spermatocytes, spermatids) and reproductive organs (epididymis, seminal vesicles, vas deferens).

Main Results:

  • cPGES expression was low at 5 days postpartum, increasing significantly by day 15 and reaching adult levels by day 20.
  • Sertoli cells showed consistently low cPGES expression from infancy to adulthood.
  • Spermatocytes and round spermatids exhibited high cPGES levels, particularly after the onset of meiosis and into adulthood.
  • High cPGES expression was also observed in the epididymis, seminal vesicles, and vas deferens.

Conclusions:

  • cPGES in Sertoli cells contributes to basal prostaglandin E2 (PGE2) synthesis in the seminiferous tubules of developing mice.
  • Elevated cPGES in spermatocytes and spermatids suggests a critical role in maintaining high PGE2 levels, potentially linked to the spermatogenic cycle and male fertility.