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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Conservation of Protein Domains02:26

Conservation of Protein Domains

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

Computational reconstruction of multidomain proteins using atomic force microscopy data.

Minh-Hieu Trinh1, Michael Odorico, Michael E Pique

  • 1CEA, iBEB, Department of Biochemistry and Nuclear Toxicology, F-30207 Bagnols sur Cèze, France.

Structure (London, England : 1993)
|January 17, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a new method combining atomic force microscopy (AFM) images with protein structures to build large macromolecular assemblies. This approach enhances AFM resolution for atomic-level structural biology insights.

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Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

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Related Experiment Videos

Last Updated: May 25, 2026

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
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Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Area of Science:

  • Structural Biology
  • Biophysics
  • Biochemistry

Background:

  • Classical structural biology methods struggle with large, flexible macromolecules.
  • High-resolution atomic detail is crucial for understanding macromolecular function.

Purpose of the Study:

  • To develop a novel computational methodology for assembling large macromolecules.
  • To integrate atomic force microscopy (AFM) topographic data with atomic coordinates for structural modeling.

Main Methods:

  • A two-step protocol involving docking protein domains and combinatorial assembly.
  • Validation using simulated antibody structures, Tobacco mosaic virus, and Aquaporin Z.

Main Results:

  • Demonstrated the utility of AFM topography and surface data as constraints for building macromolecular assemblies.
  • Successfully applied the protocol to both simulated and experimentally derived systems.

Conclusions:

  • The novel method effectively increases AFM resolution to atomic detail.
  • Applicable to diverse multicomponent and disjoint macromolecular structures.