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Related Concept Videos

Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Related Experiment Video

Updated: May 25, 2026

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
19:45

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection

Published on: October 6, 2012

Engineering stem cell expansion.

Irwin D Bernstein1, Colleen Delaney

  • 1Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA 98109, USA. lbernste@fhcrc.org

Cell Stem Cell
|February 7, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel culture method to improve the expansion of hematopoietic stem and progenitor cells ex vivo. This technique overcomes limitations by diluting inhibitory factors, enhancing therapeutic precursor generation.

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16:04

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Published on: October 8, 2009

Area of Science:

  • Stem cell biology
  • Hematopoiesis
  • Cell culture technology

Background:

  • Ex vivo expansion of hematopoietic stem/progenitor cells (HSPCs) is crucial for cell therapies.
  • Current methods face limitations in maintaining stem cell function and achieving sufficient cell numbers.
  • Accumulation of inhibitory signaling factors can impede HSPC proliferation.

Purpose of the Study:

  • To develop an improved culture method for ex vivo expansion of HSPCs.
  • To overcome the limitations of existing cell expansion techniques.
  • To enhance the generation of therapeutically relevant hematopoietic precursors.

Main Methods:

  • Development of a novel culture system for HSPC expansion.
  • Continuous dilution of inhibitory signaling molecules within the culture.
  • Maintenance of optimal stem cell density during culture.

Main Results:

  • Successfully overcame limitations in ex vivo HSPC expansion.
  • Demonstrated enhanced generation of hematopoietic precursors.
  • The novel method supports sustained stem cell function and proliferation.

Conclusions:

  • The developed culture method offers a significant advancement for ex vivo HSPC expansion.
  • This approach holds promise for improving cell-based therapies.
  • Further research can explore the therapeutic utility of the generated precursors.