Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lamin B1 safeguards the B cell genome and shapes lymphoma outcome.

HemaSphere·2026
Same author

Nanoscopic analysis of tight junction organization in in vitro blood-brain barrier models.

Fluids and barriers of the CNS·2026
Same author

Human DRG Glucocorticoid Receptor Profiling Reveals Targets for Regionally Delivered Steroid Analgesia.

Cells·2026
Same author

Single-molecule localization microscopy imaging of extracellular vesicle DNA in recipient cells.

Journal of translational medicine·2026
Same author

Gα<sub>i2</sub> Signaling Regulates Neonatal Respiratory Adaptation.

International journal of molecular sciences·2025
Same author

Cell aging - a relevant factor in live cell microscopy (mini-review).

Progress in biophysics and molecular biology·2025
Same journal

Thymidylate synthase inhibitory drugs induce p53-dependent pathways differently.

PloS one·2026
Same journal

Top-down and bottom-up attention for joint pattern classification and reconstruction.

PloS one·2026
Same journal

Short- and long-term scaling behavior of blood pressure and pulse arrival time during sleep in healthy controls and patients with obstructive sleep apnea.

PloS one·2026
Same journal

Double DQN-based secrecy energy efficiency and fairness performance in IRS-assisted NOMA systems with friendly jamming.

PloS one·2026
Same journal

10 recommendations for strengthening citizen science for improved societal and ecological outcomes: A co-produced analysis of challenges and opportunities in the 21st century.

PloS one·2026
Same journal

Paying in public: Peer effects, impression management, and willingness to pay on digital payment platforms.

PloS one·2026
See all related articles

Related Experiment Video

Updated: May 25, 2026

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
07:20

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Published on: February 18, 2022

Visualization and quantitative analysis of reconstituted tight junctions using localization microscopy.

Rainer Kaufmann1, Jörg Piontek, Frederik Grüll

  • 1Kirchhoff Institute for Physics, University of Heidelberg, Heidelberg, Germany.

Plos One
|February 10, 2012
PubMed
Summary
This summary is machine-generated.

Researchers visualized tight junction strands using advanced microscopy, revealing mesh structures that control tissue barrier permeability. This method enhances understanding of how claudins form these crucial cellular networks.

More Related Videos

Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

Related Experiment Videos

Last Updated: May 25, 2026

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
07:20

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Published on: February 18, 2022

Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

Area of Science:

  • Cell biology
  • Biophysics
  • Molecular medicine

Background:

  • Tight junctions (TJ) are critical for regulating paracellular permeability in tissue barriers.
  • Claudins (Cld) form the structural backbone of TJ strands, influencing barrier function.
  • Understanding TJ strand morphology is key to deciphering solute and macromolecule permeability.

Purpose of the Study:

  • To establish a novel localization microscopy approach for investigating TJ strand morphology.
  • To analyze the morphometric properties of TJ networks formed by Claudin 3 and Claudin 5.
  • To correlate TJ strand structure with paracellular barrier function.

Main Methods:

  • Stable transfection of HEK293 cells with Claudin 3 or Claudin 5.
  • Spectral Position Determination Microscopy (SPDM) for high-resolution imaging of TJ strands.
  • Quantitative morphometric analysis of TJ networks and single molecule distribution.

Main Results:

  • Visualization of extended TJ networks formed by Claudin 3 and Claudin 5 with ~50 nm resolution.
  • Identification of two distinct populations of elliptic meshes within TJ strands.
  • Demonstration that molecule density is significantly higher within strands compared to surrounding areas.

Conclusions:

  • The novel SPDM method provides unprecedented insights into TJ strand organization.
  • TJ strand morphology, characterized by mesh size and continuity, is a key determinant of paracellular permeability.
  • This approach facilitates a deeper mechanistic understanding of TJ protein function and tissue barrier regulation.