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Updated: May 24, 2026
Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification
Published on: September 8, 2023
Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification
Jin-Long Yang1, Rui Yang, Ke-Fei Shen
1Chongqing Academy of Animal Science, Chongqing 402460, China.
Background:
Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required.
Findings:
A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green.
Conclusions:
The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.
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