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Cell preparation for flow cytometry

M G Ormerod1

  • 1Institute of Cancer Research, The Haddow Laboratories, Sutton, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed

Abstract:

For flow cytometry, a suspension of single cells, free of large clumps and excess debris, is essential. The quality of the data obtained depends as much on the quality of the preparation as on that of the flow cytometer.

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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