Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enzyme-antienzyme method for immunohistochemistry.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Flow cytometric analysis of cells using an antibody to a surface antigen.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Multiple immunofluorescence analysis of cells using flow cytometry.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Enzyme-antienzyme method for immunohistochemistry.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Cell preparation for flow cytometry.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Flow cytometric analysis of cells using an antibody to a surface antigen.

Methods in molecular biology (Clifton, N.J.)·2012
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 16, 2026

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
08:31

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

Published on: November 4, 2022

Cell preparation for flow cytometry.

M G Ormerod1

  • 1Institute of Cancer Research, The Haddow Laboratories, Sutton, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

High-quality cell preparation is crucial for reliable flow cytometry data. Ensuring a single-cell suspension free of debris maximizes experimental accuracy.

More Related Videos

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
12:34

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

Published on: July 6, 2014

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

Related Experiment Videos

Last Updated: May 16, 2026

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
08:31

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

Published on: November 4, 2022

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
12:34

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

Published on: July 6, 2014

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

Area of Science:

  • Cellular Biology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Flow cytometry is a powerful technique for analyzing single cells.
  • Accurate analysis relies heavily on the quality of the cell sample presented to the instrument.

Purpose of the Study:

  • To emphasize the critical role of sample preparation in flow cytometry.
  • To highlight the impact of cell suspension quality on data integrity.

Main Methods:

  • Focus on the necessity of achieving a single-cell suspension.
  • Discuss the importance of removing large cell clumps and debris.

Main Results:

  • Data quality in flow cytometry is directly correlated with the quality of the cell preparation.
  • Suboptimal sample preparation leads to inaccurate or uninterpretable flow cytometry data.

Conclusions:

  • Proper cell suspension preparation is as vital as the flow cytometer itself for successful experiments.
  • Researchers must prioritize meticulous sample handling to obtain meaningful flow cytometry results.