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Updated: May 24, 2026

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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
Published on: December 18, 2014
Flow cytometric analysis of cells using an antibody to a surface antigen
1Institute of Cancer Research, The Haddow Laboratories, Sutton, Surrey, UK.
Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
Abstract:
Identifying a subset of cells, particularly peripheral blood lymphocytes, by means of a fluorescently-tagged antibody is one of the commonest applications of flow cytometry. There are many methods, all of them basically similar, for labeling antigens on cell surfaces. Two slightly different procedures are given in this chapter; these can be adapted to meet most needs.

