Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Single-Nanoparticle Dynamics in Opto-Thermal Tweezers: Resolving the Temporal Resolution of Depletion Force Trapping.

ACS nano·2026

Same author

Magnetic Nanoparticles as Label-Free Dual-Function Nanoheaters and Nanothermometers.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Controlling the sign of optical forces using metaoptics.

Nature communications·2026

Same author

Scalable Multiparametric Characterization of Aptamer-Target Interactions.

ACS nano·2026

Same author

Three-dimensional optofluidic control using reconfigurable thermal barriers.

Nature photonics·2025

Same author

Cooling of an Optically Levitated Nanoparticle via Measurement-Free Coherent Feedback.

Physical review letters·2025

Same journal

Intrinsic Superconducting Gap in Bilayer KCa<sub>2</sub>Fe<sub>4</sub>As<sub>4</sub>F<sub>2</sub> and Decoupled Monolayer FeAs.

Nano letters·2026

Same journal

Programmable Hydrogen-Assisted Chemical Vapor Deposition Growth and Bipolar Transport in Two-Dimensional MoO<sub>2</sub> Nanoflakes.

Nano letters·2026

Same journal

A Curvature-Modulated Strategy for Single-Atom Catalysts toward Reciprocal Regulation in Li-S Batteries.

Nano letters·2026

Same journal

Vacuum Pyrolysis Engineered CoSb/C Scaffold for Sodium Metal Anodes with Sodiophilic and Superionic Interphase.

Nano letters·2026

Same journal

Hexagonal SiGe Quantum Dots in Nanowires.

Nano letters·2026

Same journal

Monolithic Axial InGaAs Quantum Dot Emitters in GaAs-Based Nanowires via Sb-Mediated Facet Engineering.

Nano letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 24, 2026

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Published on: July 30, 2018

Mapping intracellular temperature using green fluorescent protein.

Jon S Donner¹, Sebastian A Thompson, Mark P Kreuzer

¹ICFO-Institut de Ciencies Fotoniques, Mediterranean Technology Park, 08860 Castelldefels (Barcelona), Spain.

|March 8, 2012

Summary

This summary is machine-generated.

Researchers developed a novel method using green fluorescent proteins (GFP) to map intracellular temperature with high accuracy. This noninvasive technique offers new possibilities for studying cellular processes and developing medical applications.

More Related Videos

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

Related Experiment Videos

Last Updated: May 24, 2026

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Published on: July 30, 2018

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

Area of Science:

Cellular biology
Biophysics
Nanotechnology

Background:

Accurate temperature monitoring is crucial for understanding cellular processes like metabolism, division, and gene expression.
Noninvasive intracellular temperature mapping can advance biological and medical research.

Purpose of the Study:

To develop and validate a noninvasive method for intracellular temperature mapping using green fluorescent proteins (GFP).
To assess the feasibility of using GFP as thermal nanoprobes for high-resolution temperature measurements within cells.

Main Methods:

Utilized fluorescence polarization anisotropy of GFP to probe temperature changes.
Employed photothermal heating of gold nanorods to induce controlled temperature variations in GFP-transfected HeLa and U-87 MG cancer cell lines.
Achieved intracellular temperature mapping with nanoscale spatial resolution.

Main Results:

Demonstrated successful intracellular temperature mapping using GFP nanoprobes.
Achieved a spatial resolution of 300 nm and a temperature accuracy of approximately 0.4 °C.
Validated the method in relevant cancer cell lines.

Conclusions:

Green fluorescent proteins (GFP) can serve as effective thermal nanoprobes for intracellular temperature mapping.
The developed method is noninvasive, highly accurate, and compatible with widely used GFP-transfected cells.
This approach offers a valuable tool for fundamental and applied research in molecular biology, medicine, and diagnostics.