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Related Concept Videos

Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Fluorescence correlation spectroscopy.

Jonas Ries1, Petra Schwille

  • 1Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|March 15, 2012
PubMed
Summary
This summary is machine-generated.

Fluorescence correlation spectroscopy (FCS) measures biomolecule properties in biological systems. This review covers FCS principles, applications, limitations, and future technical advancements.

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Area of Science:

  • Biophysics
  • Biochemistry
  • Cell Biology

Background:

  • Fluorescence correlation spectroscopy (FCS) is a sensitive method for analyzing fluorescently labeled molecules.
  • It provides insights into concentration, diffusion, and interactions of biomolecules.
  • FCS is applicable across diverse biological environments, from solutions to living organisms.

Purpose of the Study:

  • To introduce the fundamental principles of FCS.
  • To discuss the broad applications of FCS in biological research.
  • To highlight the limitations and challenges associated with FCS techniques.

Main Methods:

  • Review of the core concepts of Fluorescence Correlation Spectroscopy.
  • Analysis of existing literature on FCS applications in various biological systems.
  • Examination of recent technical innovations and future prospects in fluorescence fluctuation spectroscopy.

Main Results:

  • FCS enables quantitative measurements of biomolecular parameters.
  • The technique is versatile, applicable to homogeneous solutions, cellular environments, membranes, and whole organisms.
  • Current limitations are being addressed by novel technical developments.

Conclusions:

  • FCS is a powerful and adaptable tool for biological investigations.
  • Ongoing technical advancements promise to expand the scope and precision of FCS.
  • Future applications are expected to further enhance our understanding of complex biological processes.