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Updated: May 23, 2026

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Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
Published on: December 20, 2019
Mouse hepatocytes retain the expression of the main differentiation markers during culturing on collagen-chitosan
A V Eremeev1, A V Svetlakov, I N Bolshakov
1Center of Reproductive Medicine, Department of Operative Surgery with Topographic Anatomy, Laboratory of Cell Technologies, V. F. Voino-Yasenetsky State Medical University, Ministry of Health and Social Development, Krasnoyarsk, Russia.
Bulletin of Experimental Biology and Medicine
|March 31, 2012
Abstract:
Mouse hepatocytes cultured on artificial 3D collagen-chitosan biopolymer matrices retained the expression of hepatocyte markers for 14 days.

