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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Related Experiment Video

Updated: May 23, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Acoustofluidics 11: Affinity specific extraction and sample decomplexing using continuous flow acoustophoresis.

Per Augustsson1, Thomas Laurell

  • 1Department of Measurement Technology and Industrial Electrical Engineering, Division of Nanobiotechnology, Lund University, Sweden. per.augustsson@elmat.lth.se

Lab on a Chip
|April 3, 2012
PubMed
Summary
This summary is machine-generated.

Acoustic standing wave technology enables affinity-based selection of analytes in microfluidics. This acoustofluidic method allows for efficient carrier fluid exchange, outperforming traditional methods for sample preparation.

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Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles
10:14

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Published on: March 6, 2016

Area of Science:

  • Biotechnology
  • Microfluidics
  • Analytical Chemistry

Background:

  • Traditional sample preparation methods like magnetic bead-based extraction and centrifugation can be time-consuming and may require complex protocols.
  • Microfluidic systems offer advantages in handling small sample volumes and enabling precise control over fluid dynamics.
  • Acoustic forces present a non-invasive method for manipulating particles and cells within microfluidic devices.

Purpose of the Study:

  • To introduce and explain the principles of acoustofluidics for affinity-based sample preparation in microfluidic devices.
  • To demonstrate the capability of acoustic standing waves for carrier fluid exchange and analyte selection.
  • To provide a tutorial on fluidic configurations and system parameters impacting acoustophoresis-based affinity extraction and cell medium exchange.

Main Methods:

  • Utilizing acoustic standing wave technology combined with ligand-complexed microbeads for affinity-specific selection of target analytes.
  • Implementing acoustofluidic principles within a microfluidic format to leverage laminar flow and acoustic forces.
  • Designing fluidic configurations for acoustophoresis-based sample decomplexing and carrier fluid exchange in continuous flow.

Main Results:

  • Demonstrated successful carrier fluid exchange operations in microfluidic chips solely based on acoustofluidic properties.
  • Achieved efficient affinity-specific selection of target analytes, including microbes and molecular species.
  • Showcased potential for simpler processing protocols that may outperform macroscale methods and integrate with downstream instrumentation.

Conclusions:

  • Acoustofluidics provides an effective platform for affinity-based sample preparation and carrier fluid exchange in microfluidic systems.
  • The described methods offer a promising alternative to conventional sample processing techniques, enabling streamlined workflows.
  • This technology facilitates targeted extraction and selective elution, with potential for integration into various analytical applications.