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[Screening for genes associated with cardiac fibrosis induced by aldosterone]
Jin-Song Chen1, Qun Mo, Yong-Jin Xie
1Department of Cardiology, PLA General Hospital, Beijing 100853, China. cjsong001@sina.com
Aim:
To investigate differently expressed genes associated with cardiac fibrosis induced independently by aldosterone.
Methods:
Fetal cardiac fibroblasts (FCFs)were isolated and cultured. Total RNA was extracted 8 hours after aldosterone administration. Then gene chips were used to screen these RNA samples. Some of candidate genes were confirmed by RT-PCR and Western blot.
Results:
Differently expressed 1519 genes were screened. Up-regulated genes were 714 while down-regulated genes were 805. The expression of CCL7, MMP-26 and IL31RA was tested by RT-PCR and western blot, the results is identical with those by gene chips.
Conclusion:
Gene chip can efficiently single out differently expressed genes induced dependently by aldosterone in FCFs. CCL7, MMP-26 and IL31RA may be associated with cardiac fibrosis induced by aldosterone.
