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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...

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Updated: May 23, 2026

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
09:23

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Published on: March 7, 2017

Self-renewing endodermal progenitor lines generated from human pluripotent stem cells.

Xin Cheng1, Lei Ying, Lin Lu

  • 1Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, PA 19104, USA.

Cell Stem Cell
|April 10, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed novel endodermal progenitor (EP) cell lines from human pluripotent stem cells. These nontumorigenic EP cells can generate various endodermal tissues, offering a safe source for cell therapies and disease modeling.

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Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids
08:13

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

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Last Updated: May 23, 2026

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
09:23

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Published on: March 7, 2017

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids
08:13

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

Area of Science:

  • Stem cell biology
  • Developmental biology
  • Regenerative medicine

Background:

  • Human pluripotent stem cells (hPSCs) have limitations for research and therapy due to tumor formation and differentiation challenges.
  • Generating pure, functional cell populations from hPSCs in vitro remains a significant hurdle.

Purpose of the Study:

  • To establish and characterize novel endodermal progenitor (EP) cell lines from hPSCs.
  • To assess the self-renewal capacity, differentiation potential, and safety of these EP cells.

Main Methods:

  • Generation of EP cell lines from human embryonic stem cells and induced pluripotent stem cells.
  • Optimization of culture conditions for sustained EP cell self-renewal and maintenance of definitive endoderm characteristics.
  • In vitro differentiation assays and in vivo transplantation studies in mice.

Main Results:

  • Established EP cell lines with high self-renewal capacity (>10^16) and definitive endoderm gene expression.
  • Demonstrated differentiation of EP cells into multiple endodermal lineages, including pancreatic beta cells, hepatocytes, and intestinal epithelia.
  • Confirmed the nontumorigenic nature of EP cells in vivo.

Conclusions:

  • EP cells are a robust and safe tool for studying endoderm development.
  • These EP cells provide a promising, safe source for generating endodermal tissues for transplantation therapies.