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Updated: May 23, 2026
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
Published on: August 15, 2011
Micro in utero electroporation for efficient gene targeting in mouse embryos
1Critical Period Mechanisms Research Group, Institute of Physical and Chemical Research (RIKEN), Brain Science Institute, Saitama 351-0198, Japan.
Abstract:
INTRODUCTION A mixture of DNA and tracking dye is microinjected into a precisely targeted area of the developing mouse embryo. A pulse generator is used to pass current into the tissue using needle electrodes placed on either side of the injection. Fiberoptic light is used to visualize the procedure. This method can be readily adapted for use on most parts of the embryo. It can be used with many different types of genes, as well as for simultaneously delivering multiple genes.
