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Updated: May 23, 2026

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Multifocal multiphoton endoscope.

David R Rivera¹, Christopher M Brown, Dimitre G Ouzounov

¹School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA. drr66@cornell.edu

|April 20, 2012

Summary

This summary is machine-generated.

Researchers developed a compact multifocal multiphoton endoscope using a multi-fiber scanner. This innovation enables simultaneous image acquisition at multiple depths, advancing in vivo imaging capabilities.

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A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

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Area of Science:

Biomedical Optics
Medical Imaging
Endoscopy

Background:

Multiphoton microscopy offers deep tissue imaging with intrinsic optical sectioning.
Traditional multiphoton endoscopes face limitations in size, flexibility, and simultaneous multi-depth imaging.
The need for compact, high-resolution endoscopic tools for longitudinal in vivo studies is critical.

Purpose of the Study:

To develop a miniaturized, flexible multifocal multiphoton endoscope.
To achieve simultaneous image acquisition at multiple axial depths.
To enable compact, high-speed, high-resolution longitudinal in vivo imaging.

Main Methods:

A miniaturized resonant/non-resonant multi-fiber raster scanner was integrated with a gradient-index lens assembly.
Three axially offset double-clad optical fibers were incorporated for simultaneous multi-depth imaging.
The endoscope system was characterized for imaging speed, resolution, and physical dimensions.

Main Results:

A compact endoscope with a 3 mm outer diameter and 4 cm rigid length was fabricated.
Simultaneous multiphoton imaging was achieved at three axial depths (≥4.8 μm separation).
Imaging speed reached 4 frames/s per focal plane with 0.8 μm lateral and 10 μm axial resolution (two-photon).

Conclusions:

The developed multifocal multiphoton endoscope provides a compact and flexible solution for in vivo imaging.
Simultaneous multi-depth acquisition enhances efficiency for longitudinal studies.
This technology has potential applications in neuroscience, pathology, and other biomedical research fields.