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Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
A general method for detecting protease activity via gelation and its application to artificial clotting
Steven C Bremmer1, Jing Chen, Anne J McNeil
1Department of Medicinal Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan, 48109-1055, USA.
Chemical Communications (Cambridge, England)
|April 28, 2012
Abstract:
A modular system for detecting protease activity via enzyme-triggered gel formation is described. Protease-specific recognition sequences are utilized to achieve enzyme specificity. Artificial blood clotting is demonstrated by activating endogenous thrombin to trigger gelation in fibrinogen-deficient blood plasma.
